Biological Laboratory X-ray Microscopy
نویسندگان
چکیده
منابع مشابه
Laboratory cryo soft X-ray microscopy.
Lens-based water-window X-ray microscopy allows two- and three-dimensional (2D and 3D) imaging of intact unstained cells in their near-native state with unprecedented contrast and resolution. Cryofixation is essential to avoid radiation damage to the sample. Present cryo X-ray microscopes rely on synchrotron radiation sources, thereby limiting the accessibility for a wider community of biologis...
متن کاملBiological imaging by soft x-ray diffraction microscopy.
We have used the method of x-ray diffraction microscopy to image the complex-valued exit wave of an intact and unstained yeast cell. The images of the freeze-dried cell, obtained by using 750-eV x-rays from different angular orientations, portray several of the cell's major internal components to 30-nm resolution. The good agreement among the independently recovered structures demonstrates the ...
متن کاملX-ray contact microscopy system for spectromicroscopy of biological specimens.
An X-ray contact microscopy system has been developed for the study of molecular and elemental distributions in biological specimens based on X-ray absorption characteristics. The system consists of a chamber for measuring XANES (X-ray absorption near-edge structure) of biomolecules, and a contact microscopy system for dried specimens with an electronic zooming tube. With this system the elemen...
متن کاملQuantitative biological imaging by ptychographic x-ray diffraction microscopy.
Recent advances in coherent x-ray diffractive imaging have paved the way to reliable and quantitative imaging of noncompact specimens at the nanometer scale. Introduced a year ago, an advanced implementation of ptychographic coherent diffractive imaging has removed much of the previous limitations regarding sample preparation and illumination conditions. Here, we apply this recent approach towa...
متن کاملFocused issue on X-ray microscopy of biological materials.
The study of biological structures at different scales and resolution levels requires the use of imaging methods that are appropriate for each level of analysis. As recently reviewed in this journal (Volume 172, Issue 2 (2010)), the use of multiple, correlated methods is a powerful way to extend the analytical properties of each individual technique, normally resulting in more informative insig...
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ژورنال
عنوان ژورنال: Microscopy and Microanalysis
سال: 2018
ISSN: 1431-9276,1435-8115
DOI: 10.1017/s1431927618014022